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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Immunoprecipitation [2]
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- Product number
- NB100-2310 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2310, RRID:AB_2297022
- Product name
- Rabbit Polyclonal EPLIN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition.
Zhang S, Wang X, Iqbal S, Wang Y, Osunkoya AO, Chen Z, Chen Z, Shin DM, Yuan H, Wang YA, Zhau HE, Chung LW, Ritenour C, Kucuk O, Wu D
The Journal of biological chemistry 2013 Jan 18;288(3):1469-79
The Journal of biological chemistry 2013 Jan 18;288(3):1469-79
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of Human EPLIN by Immunoprecipitation. Samples: Whole cell lysate (500 mcg) from DU145 cells. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2310 used at 10 mcg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2310 (lot NB100-2310-2) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by a previous lot of this antibody (lot NB100-2310-1) and rabbit anti-EPLIN antibody NB100-2305. Detection: Chemiluminescence with an exposure time of 3 minutes.
