Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-19438 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal mtTFA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted by 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody [NBP2-19438] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody ) diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody [NBP2-19438] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: mtTFA Antibody [NBP2-19438] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.