AP10271b

antibody from Abcepta

Targeting: TFAM TCF6, TCF6L2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP10271b

Provider

Abcepta

Proper citation

Abgent Cat#AP10271b, RRID:AB_10821048

Product name

TFAM Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysate from 293T cell line, using TFAM Antibody (C-term) (Cat. #AP10271b). AP10271b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"TFAM Antibody (C-term) (Cat. #AP10271b) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow)."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody (C-term)(Cat#AP10271b). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly."

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TFAM antibody (C-term) (Cat. #AP10271b) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAM antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TFAM Antibody (C-term) (Cat. #AP10271b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50