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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX112760 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112760, RRID:AB_10724875
- Product name
- mtTFA antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE GTX112760 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- mtTFA antibody detects mtTFA protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.Red: Mitotracker, a mitochondria marker, diluted at 1:2000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 £gg of mtTFA antibody (GTX112760) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX112760) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA(GTX112760) antibody at 1:250 dilution.
