PB9447

antibody from Boster Biological Technology

Targeting: TFAM TCF6, TCF6L2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9447

Provider

Boster Biological Technology

Product name

Anti-mtTFA/TFAM Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for MTTFA/TFAM detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. MTTFA/TFAM information: Molecular Weight: 29097 MW; Subcellular Localization: Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

ß1-adrenergic blockers preserve neuromuscular function by inhibiting the production of extracellular traps during systemic inflammation in mice.

Bourcier CH, Michel-Flutot P, Emam L, Adam L, Gasser A, Vinit S, Mansart A
Frontiers in immunology 2023;14:1228374

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AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy.

Zhu M, Shen W, Li J, Jia N, Xiong Y, Miao J, Xie C, Chen Q, Shen K, Meng P, Li X, Wu Q, Zhou S, Wang M, Kong Y, Zhou L
Frontiers in pharmacology 2022;13:836496

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CXC Chemokine Receptor 2 Accelerates Tubular Cell Senescence and Renal Fibrosis via β-Catenin-Induced Mitochondrial Dysfunction.

Meng P, Huang J, Ling X, Zhou S, Wei J, Zhu M, Miao J, Shen W, Li J, Ye H, Niu H, Zhang Y, Zhou L
Frontiers in cell and developmental biology 2022;10:862675

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Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.

Ni HY, Yu L, Zhao XL, Wang LT, Zhao CJ, Huang H, Zhu HL, Efferth T, Gu CB, Fu YJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Apr;98:153919

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Cannabinoid receptor 2 plays a central role in renal tubular mitochondrial dysfunction and kidney ageing.

Zhou S, Ling X, Meng P, Liang Y, Shen K, Wu Q, Zhang Y, Chen Q, Chen S, Liu Y, Zhou L
Journal of cellular and molecular medicine 2021 Sep;25(18):8957-8972

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Cognitive Protective Mechanism of Crocin Pretreatment in Rat Submitted to Acute High-Altitude Hypoxia Exposure.

Zhang X, Zhang X, Dang Z, Su S, Li Z, Lu D
BioMed research international 2020;2020:3409679

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of mtTFA using anti-mtTFA antibody (PB9447). mtTFA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-mtTFA Antibody (PB9447) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image