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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F40574 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TFAM Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 216-246 from the human protein was used as the immunogen for this TFAM antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the TFAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) HeLa, (2) HepG2 and (3) U-2OS cell line using TFAM antibody at 1:1000. Expected molecular weight: 24~29 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- TFAM antibody western blot analysis in (1) HeLa, (2) Jurkat, (3) K562, and (4) MCF-7 lysate; Expected molecular weight: 24~29 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 293T cell line using TFAM antibody diluted at 1:1000. Expected molecular weight: 24~29 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of NCI-H460 cell stained with TFAM antibody at 1:25. TFAM immunoreactivity is localized to mitochondrion.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- TFAM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- TFAM antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
