Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R32176 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TFAM Antibody / mtTFA
- Antibody type
- Polyclonal
- Antigen
- Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY of human mtTFA were used as the immunogen for the mtTFA antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the mtTFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) human placenta, 3) HeLa, 4) HEPA, 5) HepG2 and 6) COLO320 with mtTFA antibody. Expected/observed molecular weight 24~29 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Jurkat, 2) A431, 3) HL-60, 4) CCRF-CEM, 5) 293T and 6) SW620 lysate with mtTFA antibody at 0.5ug/ml. Expected molecular weight 24~29 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer with mtTFA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.