HPA063684

antibody from Atlas Antibodies

Targeting: TFAM TCF6, TCF6L2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA063684

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA063684, RRID:AB_2685089

Product name

Anti-TFAM

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

QEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAK
EDETRYHNEMKSWEEQMIEVGRKDLLRRTIKKQRK
YGAE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines Caco-2 and HeLa using Anti-TFAM antibody. Corresponding TFAM RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-TFAM antibody HPA063684 (A) shows similar pattern to independent antibody HPA040648 (B).

Antibody #2 product nr

HPA040648

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line RH-30 shows localization to mitochondria.

Sample type

HUMAN