Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-71648 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71648, RRID:AB_11037615
- Product name
- Mouse Monoclonal mtTFA Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells.
Exposure of Monocytic Cells to Lipopolysaccharide Induces Coordinated Endotoxin Tolerance, Mitochondrial Biogenesis, Mitophagy, and Antioxidant Defenses.
3D super-resolution microscopy reflects mitochondrial cristae alternations and mtDNA nucleoid size and distribution.
Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation.
Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-γ.
Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways.
Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency.
Brain diabetic neurodegeneration segregates with low intrinsic aerobic capacity.
PGC-1α overexpression results in increased hepatic fatty acid oxidation with reduced triacylglycerol accumulation and secretion.
Takishita Y, Yasuda H, Shimizu M, Matsuo A, Morita A, Tsutsumi T, Tsuchiya M, Sato EF
Journal of clinical biochemistry and nutrition 2020 Jan;66(1):15-23
Journal of clinical biochemistry and nutrition 2020 Jan;66(1):15-23
Exposure of Monocytic Cells to Lipopolysaccharide Induces Coordinated Endotoxin Tolerance, Mitochondrial Biogenesis, Mitophagy, and Antioxidant Defenses.
Widdrington JD, Gomez-Duran A, Pyle A, Ruchaud-Sparagano MH, Scott J, Baudouin SV, Rostron AJ, Lovat PE, Chinnery PF, Simpson AJ
Frontiers in immunology 2018;9:2217
Frontiers in immunology 2018;9:2217
3D super-resolution microscopy reflects mitochondrial cristae alternations and mtDNA nucleoid size and distribution.
Dlasková A, Engstová H, Špaček T, Kahancová A, Pavluch V, Smolková K, Špačková J, Bartoš M, Hlavatá LP, Ježek P
Biochimica et biophysica acta. Bioenergetics 2018 Sep;1859(9):829-844
Biochimica et biophysica acta. Bioenergetics 2018 Sep;1859(9):829-844
Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation.
Huang CL, Wang KC, Yang YC, Chiou CT, Tan CH, Lin YL, Huang NK
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jan 15;39:75-84
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jan 15;39:75-84
Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-γ.
Widdrington JD, Gomez-Duran A, Steyn JS, Pyle A, Ruchaud-Sparagano MH, Scott J, Baudouin SV, Rostron AJ, Simpson J, Chinnery PF
The Journal of allergy and clinical immunology 2017 Nov;140(5):1461-1464.e8
The Journal of allergy and clinical immunology 2017 Nov;140(5):1461-1464.e8
Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways.
Mastrocola R, Penna C, Tullio F, Femminò S, Nigro D, Chiazza F, Serpe L, Collotta D, Alloatti G, Cocco M, Bertinaria M, Pagliaro P, Aragno M, Collino M
Oxidative medicine and cellular longevity 2016;2016:5271251
Oxidative medicine and cellular longevity 2016;2016:5271251
Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency.
Sun Q, Zhong W, Zhang W, Zhou Z
American journal of physiology. Gastrointestinal and liver physiology 2016 Feb 1;310(3):G205-14
American journal of physiology. Gastrointestinal and liver physiology 2016 Feb 1;310(3):G205-14
Brain diabetic neurodegeneration segregates with low intrinsic aerobic capacity.
Choi J, Chandrasekaran K, Demarest TG, Kristian T, Xu S, Vijaykumar K, Dsouza KG, Qi NR, Yarowsky PJ, Gallipoli R, Koch LG, Fiskum GM, Britton SL, Russell JW
Annals of clinical and translational neurology 2014 Aug;1(8):589-604
Annals of clinical and translational neurology 2014 Aug;1(8):589-604
PGC-1α overexpression results in increased hepatic fatty acid oxidation with reduced triacylglycerol accumulation and secretion.
Morris EM, Meers GM, Booth FW, Fritsche KL, Hardin CD, Thyfault JP, Ibdah JA
American journal of physiology. Gastrointestinal and liver physiology 2012 Oct 15;303(8):G979-92
American journal of physiology. Gastrointestinal and liver physiology 2012 Oct 15;303(8):G979-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.