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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab116339 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant mtTFA Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human mtTFA Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Application:WB: 1/500-1/1000 IHC: 1/50-1/100IF/ICC: 1/50-1/200FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 57-70- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant mtTFA Antibody (Ab116339) - Western Blot All lanes: Recombinant mtTFA Antibody (Ab116339) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 11.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant mtTFA Antibody (Ab116339) - ICC/IF Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant mtTFA Antibody (Ab116339) and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant mtTFA Antibody (Ab116339) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant mtTFA Antibody (Ab116339) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
