PA5-23776

antibody from Invitrogen Antibodies

Targeting: TFAM TCF6, TCF6L2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23776

Provider

Invitrogen Antibodies

Product name

TFAM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Prevention of HIV-1 TAT Protein-Induced Peripheral Neuropathy and Mitochondrial Disruption by the Antimuscarinic Pirenzepine.

Han MM, Frizzi KE, Ellis RJ, Calcutt NA, Fields JA
Frontiers in neurology 2021;12:663373

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Mitochondrial biogenesis is altered in HIV+ brains exposed to ART: Implications for therapeutic targeting of astroglia.

Swinton MK, Carson A, Telese F, Sanchez AB, Soontornniyomkij B, Rad L, Batki I, Quintanilla B, Pérez-Santiago J, Achim CL, Letendre S, Ellis RJ, Grant I, Murphy AN, Fields JA
Neurobiology of disease 2019 Oct;130:104502

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Tenofovir disoproxil fumarate induces peripheral neuropathy and alters inflammation and mitochondrial biogenesis in the brains of mice.

Fields JA, Swinton MK, Carson A, Soontornniyomkij B, Lindsay C, Han MM, Frizzi K, Sambhwani S, Murphy A, Achim CL, Ellis RJ, Calcutt NA
Scientific reports 2019 Nov 20;9(1):17158

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SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy.

Jevtic V, Kindle P, Avilov SV
PloS one 2018;13(9):e0203956

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCI-H460 cells using a TFAM polyclonal antibody (Product # PA5-23776). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a TFAM polyclonal antibody (Product # PA5-23776) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TFAM in formalin fixed and paraffin embedded human testis carcinoma. Samples were incubated with TFAM polyclonal antibody (Product # PA5-23776) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of K562 cells using a TFAM polyclonal antibody (Product # PA5-23776) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 PGC-1alpha protein levels are increased and TFAM and DNM1L protein levels are decreased in brain lysates from wt and gp120-tg mice administered Tenofovir disproxil fumarate. ( a ) Immunoblot for PGC-1alpha, TFAM, and DNM1L using whole lysates from hippocampal region. ( b--d ) Densitometry for quantification of PGC-1alpha, and TFAM, and DNM1L normalized to total protein levels. Significance (p < 0.05) was determined by two-way ANOVA (wt, n = 8; wt + TDF, n = 5; gp120-tg, n = 7; gp120-tg, n = 7).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Mitochondrial proteins in mouse sciatic nerve. (A) Representative immunoblot for TFAM, proteins of oxidative phosphorylation complexes III, IV, and V, DNM1L and ACTB. (B) Densitometric analysis of TFAM normalized to ACTB. (C) Densitometric analysis of complex IV(MTC01 sub-unit) protein normalized to ACTB. (D) Densitometric analyses of DNM1L protein normalized to ACTB. Data are mean + SEM of N = 3 for C57Bl/6J group and N = 4/group for remaining groups. Statistical analyses by unpaired test or one-way ANOVA with Tukey's post-hoc test.