PA5-29571

antibody from Invitrogen Antibodies

Targeting: TFAM TCF6, TCF6L2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29571

Provider

Invitrogen Antibodies

Product name

TFAM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A. Predicted reactivity: Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Alcalase Potato Protein Hydrolysate-PPH902 Enhances Myogenic Differentiation and Enhances Skeletal Muscle Protein Synthesis under High Glucose Condition in C2C12 Cells.

Chen YJ, Chang CF, Angayarkanni J, Lin WT
Molecules (Basel, Switzerland) 2021 Oct 30;26(21)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TFAM was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM Polyclonal Antibody (Product # PA5-29571) diluted at 1:500. Red: Mitotracker, a mitochondria marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TFAM was performed in NT2D1 cells fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: TFAM Polyclonal Antibody (Product # PA5-29571) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody (Product # PA5-29571) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of mtTFA was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with mtTFA Rabbit Polyclonal Antibody (Product # PA5-29571) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing mitochondrial pattern of mtTFA. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TFAM Polyclonal Antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 µg of TFAM Polyclonal Antibody (Product # PA5-29571) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by TFAM Polyclonal Antibody (Product # PA5-29571) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 Effect of PPH902 on the activation of mitochondrial biogenesis: Representative Western blotting shows changes in the levels of NRF1 and TFAM in C2C12 cells. *** p < 0.001 indicates a significant difference with respect to control groups and ## p < 0.01 and ### p < 0.001 indicates a significance difference with respect to the high glucose challenge groups.