12974-1-AP

antibody from Invitrogen Antibodies

Targeting: CDC5L CDC5, CEF1, hCDC5, PCDC5RP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12974-1-AP

Provider

Invitrogen Antibodies

Product name

CDC5L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NPEDGMADY SDPSYVKQME RESREHLRLG LLGLPAPKND FEIVLPENAE KELEEREIDD TYIEDAADVD ARKQAIRDAE RVKEMKRMHK AVQKDLPRPS EVNETILRPL NVEPPLTDLQ KSEELIKKEM ITMLHYDLLH HPYEPSGNKK GKTVGFGTNN SEHITYLEHN PYEKFSKEEL KKAQDVLVQE MEVVKQGMSH GELSSEAYNQ VWEECYSQVL YLPGQSRYTR ANLASKKDRI ESLEKRLEIN RGHMTTEAKR AAKMEKKMKI LLGGYQSRAM GLMKQLNDLW DQIEQAHLEL RTFEELKKHE DSAIPRRLEC LKEDVQRQQE REKELQHRYA DLLLEKETLK SKF (451-803 aa encoded by BC001568)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12974-1-AP (CDC5L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12974-1-AP (CDC5L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:600) with HepG2 cells lysate 4000ug.