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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
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- Product number
- PA5-52286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADNP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: SGSPFDPVFE VEPKISNDNP EEHVLKVIPE DASESEEKLD QKEDGSKYET IHLTEEPTKL MHNASDSEVD QDDVVEWKDG ASPSESGPGS QQVSDFEDNT CEMKPGTWSD ESSQSEDARS SKPAAKKKAT MQGDREQLKW KNSSYGKVEG Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 88%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references KSHV episome tethering sites on host chromosomes and regulation of latency-lytic switch by CHD4.
Kumar A, Lyu Y, Yanagihashi Y, Chantarasrivong C, Majerciak V, Salemi M, Wang KH, Inagaki T, Chuang F, Davis RR, Tepper CG, Nakano K, Izumiya C, Shimoda M, Nakajima KI, Merleev A, Zheng ZM, Campbell M, Izumiya Y
Cell reports 2022 May 10;39(6):110788
Cell reports 2022 May 10;39(6):110788
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADNP in human cell line SH-SY5Y using a ADNP Polyclonal Antibody (Product # PA5-52286).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADNP in mouse cell line NIH-3T3 and rat cell line NBT-II using a ADNP Polyclonal Antibody (Product # PA5-52286).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ADNP in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an ADNP Polyclonal Antibody (Product # PA5-52286).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ADNP in human cell line U-2 OS using ADNP Polyclonal Antibody (Product # PA5-52286). Staining shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human endometrium using ADNP Polyclonal Antibody (Product # PA5-52286) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human liver using ADNP Polyclonal Antibody (Product # PA5-52286) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human prostate using ADNP Polyclonal Antibody (Product # PA5-52286) shows strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human testis using ADNP Polyclonal Antibody (Product # PA5-52286) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human endometrium using ADNP Polyclonal Antibody (Product # PA5-52286) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human liver using ADNP Polyclonal Antibody (Product # PA5-52286) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human prostate using ADNP Polyclonal Antibody (Product # PA5-52286) shows strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADNP in human testis using ADNP Polyclonal Antibody (Product # PA5-52286) shows moderate to strong nuclear positivity in cells in seminiferous ducts.
