Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-113482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADNP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.17 mg/mL
- Storage
- -20°C or -80°C if preferred
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADNP using a polyclonal antibody (Product # PA5-113482) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in U251 whole cell lysate, HL60 whole cell lysate, and 293T whole cell lysate. Observed band: 135 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADNP using a polyclonal antibody (Product # PA5-113482) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in U251 whole cell lysate, HL60 whole cell lysate, and 293T whole cell lysate. Observed band: 135 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded ADNP in human brain tissue using a ADNP polyclonal antibody (Product # PA5-113482) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). The section was blocked with 10% normal goat serum for 30 min at room temperature. The primary antibody, in 1% BSA, was incubated at 4°C overnight. A biotinylated secondary antibody was used with an HRP conjugated SP system.
