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LearnPA5-20455
antibody from Invitrogen Antibodies
Targeting: ZBP1
C20orf183, DAI, dJ718J7.3, DLM-1, DLM1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-20455 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive controls are mouse small intestine or human small intestine tissue lysate. PA5-20455 can be used with blocking peptide PEP-0572. PA5-20455 detects ZBP1 in WB application with human samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C
Submitted references The innate sensor ZBP1-IRF3 axis regulates cell proliferation in multiple myeloma.
Ponnusamy K, Tzioni MM, Begum M, Robinson ME, Caputo VS, Katsarou A, Trasanidis N, Xiao X, Kostopoulos IV, Iskander D, Roberts I, Trivedi P, Auner HW, Naresh K, Chaidos A, Karadimitris A
Haematologica 2022 Mar 1;107(3):721-732
Haematologica 2022 Mar 1;107(3):721-732
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZBP1 in mouse small intestine tissue lysate with ZBP1 Polyclonal Antibody (Product # PA5-20455) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBP1 was performed by loading 30 µg of THP-1 lysate in reducing sample buffer onto a 4-15% Precast Protein Gels. Proteins were transferred to PVDF membrane and followed by blocking in TBST+5% non-fat milk for one hour at room temperature. ZBP1 was detected at approximately 46 kDa using a ZBP1 polyclonal antibody (Product # PA5-20455) at a dilution of 1:500 overnight at 4°C on a rocking platform, followed by a Rabbit IgG Horseradish Peroxidase-conjugated Antibody at a dilution of 1:1,000 for one hour. Chemiluminescent detection was performed using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080) and ImageQuant LAS 4000 (GE, 28-9558-10).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ZBP1 in human small intestine tissue with ZBP1 Polyclonal Antibody (Product # PA5-20455) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZBP1 in Human Small Intestine tissue with ZBP1 Polyclonal Antibody (Product # PA5-20455) at 20 µgg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ZBP1 in Human Small Intestine tissue with ZBP1 Polyclonal Antibody (Product # PA5-20455) at 20 µgg/mL.
