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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA500787 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500787, RRID:AB_11127163
- Product name
- Anti-HDAC10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)
- Antibody type
- Monoclonal
- Description
- Anti-HDAC10 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HDAC10
- Isotype
- IgG
- Antibody clone number
- OTI5E2
- Vial size
- 100 µl
- Concentration
- 0.9 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of HDAC10 by using TrueMab monoclonal anti-HDAC10 antibody (Negative control: IP without adding anti-HDAC10 antibody). For each experiment, 500ul of DDK tagged HDAC10 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HDAC10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500787, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HDAC10mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500787, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-HDAC10 antibody(TA500787),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
