Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Protease-Activated Receptor-4 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Protease-Activated Receptor-4 (extracellular) in human breast adenocarcinoma cell line. A) Samples were probed with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) at a dilution of 1:50, and incubated with goat anti-rabbit-AlexaFluor-488 and Hoechst. B) Live view image. C) Nuclei stained image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protease-Activated Receptor-4 (extracellular) in paraffin-embedded rat lung. Samples were probed with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) using a dilution of 1:100, and incubated with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Protease-Activated Receptor-4 (extracellular) in paraffin-embedded rat lung. Samples were probed with Protease-Activated Receptor-4 (extracellular) polyclonal antibody (Product # PA5-77686) using a dilution of 1:100, and incubated with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human promyelocytic leukemia HL-60 cells using polyclonal Protease-Activated Receptor-4 (extracellular) antibody (Product # PA5-77686) with a dilution of 1:40 followed by a goat-anti-rabbit-FITC secondary antibody.