659702
antibody from BioLegend
Targeting: NLRC4
CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, ipaf
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 659702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#659702, RRID:AB_2563347
- Product name
- Purified anti-NLRC4 (IPAF)
- Antibody type
- Monoclonal
- Antigen
- Partial human NLRC4 recombinant protein (1-88 aa)
- Description
- NLRC4 , also known as IPAF, is a member of the NOD-like receptor (NLR) family. The NLR proteins share a conserved DNA-binding NACHT domain, a caspase recruitment domain (CARD), and a leucine-rich repeat (LRR) domain responsible for pathogen pattern recognition. NLRC4 was found to play an important role in the innate immune response against bacterial infection. During Gram-negative bacteria infections, such as Shigella, Salmonella, Legionella, or Pseudomonas, NLRC4 detects and is activated by translocated bacterial flagellin or PrgJ-like rod proteins subsequently resulting in the assembly of the NLRC4 inflammasome. The activated inflammasome drives proteolytic activation of caspase-1, leading to the processing and secretion of the proinflamatory cytokines IL-1β and IL-18.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 6H9B13
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysate from THP-1 cells was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRC4 (clone 6H9B13) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated