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LearnPA5-54228
antibody from Invitrogen Antibodies
Targeting: NSUN5
FLJ10267, NOL1R, NSUN5A, p120(NOL1), WBSCR20, WBSCR20A, Ynl022cL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-54228 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: QLPRFVRVNT LKTCSDDVVD YFKRQGFSYQ GRASSLDDLR ALKGKHFLLD PLMPELLVFP AQTDLHEHPL YRAGHLIL Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSUN5 in human cell lines Caco-2 and U-251MG using a NSUN5 Polyclonal Antibody (Product # PA5-54228). Corresponding NSUN5 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NSUN5 in human cell line A-431 shows positivity in nucleus & nucleoli. Samples were probed using a NSUN5 Polyclonal Antibody (Product # PA5-54228).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of NSUN5 in human cell line A-431 using NSUN5 Polyclonal Antibody (Product # PA5-54228). Staining shows positivity in nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NSUN5 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal NSUN5 antibody (Product # PA5-54228) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NSUN5 in human rectum using NSUN5 Polyclonal Antibody (Product # PA5-54228) shows strong nuclear and cytoplasmic positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNA immunoprecipitation (RIP) western of NSUN5 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal NSUN5 antibody (Product # PA5-54228) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNA immunoprecipitation (RIP) western of NSUN5 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of polyclonal NSUN5 antibody (Product # PA5-54228) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.
