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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-27135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EAR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, Raji, NIH-3T3, JC. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EAR2 using 30 µg of A) NIH-3T3 and B) JC lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EAR2 polyclonal antibody (Product # PA5-27135) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EAR2 using 30 µg of A) HeLa and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EAR2 polyclonal antibody (Product # PA5-27135) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EAR2 Polyclonal Antibody (Product # PA5-27135). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with EAR2 Polyclonal Antibody (Product # PA5-27135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using EAR2 (Product # PA5-27135) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
