Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOXA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 37 kDa.
- Concentration
- 1 mg/mL
Submitted references Identification of HOXA1 as a Novel Biomarker in Prognosis of Head and Neck Squamous Cell Carcinoma.
Li H, Wang X, Zhang M, Wang M, Zhang J, Ma S
Frontiers in molecular biosciences 2020;7:602068
Frontiers in molecular biosciences 2020;7:602068
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOXA1 using HOXA1 polyclonal antibody (Product # PA5-36164) at a dilution of 1:500. Lane 1: Hela whole cell lysate, Lane 2: sp2/0 whole cell lysate, Lane 3: PC12 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HOXA1 in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with HOXA1 polyclonal antibody (Product # PA5-36164) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Transcriptional expression and protein level of HOXA1 is significantly high in HNSCC patients. (A) Higher HOXA1 mRNA expression in 499 tumor tissues compared with 43 para-carcinoma tissues based on TCGA-HNSC DATA ( p = 1.241e-10). (B) Paired difference analysis of HOXA1 mRNA expression based on TCGA-HNSC DATA ( p = 9.984e-09). (C) The percentage of HOXA1-positive area was significantly higher in HNSCC sections than in para-carcinoma sections ( P < 0.0001). (D) The percentage of HOXA1-positive cells was significantly higher in HNSCC sections than in para-carcinoma sections ( P < 0.0001). (E-G) Representative immunohistochemical staining for HOXA1 protein in 52 matched HNSCC tissues and para-carcinoma tissues. (E) Negative expression of HOXA1 in para-carcinoma tissues (-). (F) Low expression of HOXA1 protein in HNSCC specimens (- to +). (G) High expression of HOXA1 protein in primary HNSCC specimens (++). Original magnification: x200 (top), x400 (bottom).
