H00006300-M01

antibody from Abnova Corporation

Targeting: MAPK12 ERK6, p38gamma, PRKM12, SAPK-3, SAPK3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006300-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006300-M01, RRID:AB_425669

Product name

MAPK12 monoclonal antibody (M01), clone 4F4-3D2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAPK12.

Antigen sequence

MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGS
GAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKR
AYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDF
YLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKG
LRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLA
RQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIW
SVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTP
PAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNA
SPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLH
DTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLS
FKPPRQLGARVSKETPL


Isotype

IgG

Antibody clone number

4F4-3D2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAPK12 expression in transfected 293T cell line by MAPK12 monoclonal antibody (M01), clone 4F4-3D2.Lane 1: MAPK12 transfected lysate(41.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAPK12 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol