A300-446A

antibody from Invitrogen Antibodies

Targeting: BAZ1B WBSCR10, WBSCR9, WSTF

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-446A

Provider

Invitrogen Antibodies

Product name

WSTF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, HEK293T, HCT 116, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WSTF antibody (Product # A300-446A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WSTF antibody A300-446A (lot A300-446A-2) used for IP at 3 µg per reaction. WSTF was also immunoprecipitated by a previous lot of this antibody (lot A300-446A-1). For blotting immunoprecipitated WSTF, A300-446A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by western blot. Samples: Whole cell lysate (50 µg) from HCT116, HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WSTF antibody A300-446A (lot A300-446A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by immunohistochemistry. Sample: FFPE section of human breast cancer. Antibodies: Affinity purified rabbit anti-WSTF (Product # A300-446A; lot 3) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-WSTF (Cat. No. A300-446A Lot 2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WSTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WSTF antibody (Product # A300-446A lot 3) used for IP at 6 µg per reaction. WSTF was also immunoprecipitated by a previous lot of this antibody (Product # A300-446A lot 2). For blotting immunoprecipitated WSTF (Product # A300-446A) was used at 0.2 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.