Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB600-279 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-279, RRID:AB_2064677
- Product name
- Rabbit Polyclonal WSTF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Non-canonical reader modules of BAZ1A promote recovery from DNA damage.
Identification of proteins at active, stalled, and collapsed replication forks using isolation of proteins on nascent DNA (iPOND) coupled with mass spectrometry.
Oppikofer M, Sagolla M, Haley B, Zhang HM, Kummerfeld SK, Sudhamsu J, Flynn EM, Bai T, Zhang J, Ciferri C, Cochran AG
Nature communications 2017 Oct 11;8(1):862
Nature communications 2017 Oct 11;8(1):862
Identification of proteins at active, stalled, and collapsed replication forks using isolation of proteins on nascent DNA (iPOND) coupled with mass spectrometry.
Sirbu BM, McDonald WH, Dungrawala H, Badu-Nkansah A, Kavanaugh GM, Chen Y, Tabb DL, Cortez D
The Journal of biological chemistry 2013 Nov 1;288(44):31458-67
The Journal of biological chemistry 2013 Nov 1;288(44):31458-67
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WSTF Antibody [NB600-279] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP) from HeLa cells. Affinity purified rabbit anti-WSTF antibody NB600-279 used at 0.05 ug/ml for WB and at 3 ug/mg lysate for IP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WSTF Antibody [NB600-279] - Whole cell lysate (50 ug) from HCT116, HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WSTF antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: WSTF Antibody [NB600-279] - Detection of human WSTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WSTF antibody NB600-279(lot NB600-279-2) used for IP at 3 ug per reaction. WSTF was also immunoprecipitated by a previous lot of this antibody (lot NB600-279-1). For blotting immunoprecipitated WSTF, NB600-279 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WSTF Antibody [NB600-279] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-WSTF used at a dilution of 1:200 (1ug/ml). Detection: DAB