12486-1-AP

antibody from Invitrogen Antibodies

Targeting: CYP46A1 CYP46

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12486-1-AP

Provider

Invitrogen Antibodies

Product name

CYP46A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TSMLLGAQK PLSQAVKLML EGITASRNTL AKFLPGKRKQ LREVRESIRF LRQVGRDWVQ RRREALKRGE EVPADILTQI LKAEEGAQDD EGLLDNFVTF FIAGHETSAN HLAFTVMELS RQPEIVARLQ AEVDEVIGSK RYLDFEDLGR LQYLSQVLKE SLRLYPPAWG TFRLLEEETL IDGVRVPGNT PLLFSTYVMG RMDTYFEDPL TFNPDRFGPG APKPRFTYFP FSLGHRSCIG QQFAQMEVKV VMAKLLQRLE FRLVPGQRFG LQEQATLKPL DPVLCTLRPR GWQPAPPPPP C (201-500 aa encoded by BC022539)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12486-1-AP (CYP46A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12486-1-AP (CYP46A1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 12486-1-AP (CYP46A1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 12486-1-AP) (CYP46A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CYP46A1 (IP:12486-1-AP, 4ug; Detection:12486-1-AP 1:500) with mouse brain tissue lysate 3200ug.