PA1-16806
antibody from Invitrogen Antibodies
Targeting: MLXIPL
bHLHd14, CHREBP, MIO, MONDOB, WBSCR14, WS-bHLH
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA1-16806 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ChREBP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of ChREBP in 20 µg of human hepatocyte lysate using Product # PA1-16806. 5-10 second film exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of ChREBP in liver nuclear extracts from well-fed rats. 7% SDS gel, 1:1,000 dilution of Product # PA1-16806.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ChREBP in 20 µg human hepatocyte lysate. Samples were incubated in ChREBP polyclonal antibody (Product # PA1-16806). 5-10 second film exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ChREBP Polyclonal Antibody, (Product # PA1-16806) and a 120 kDa band corresponding to ChREBP was observed in the tissues tested with liver showing higher expression when compared to heart. Whole cell extracts (40 µg lysate) of Mouse liver (Lane 1), Mouse heart (Lane 2), Rat liver (Lane 3) and Rat heart (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of a human hepatocyte using Product # PA1-16806. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ChREBP in mouse liver. Samples were incubated with ChREBP polyclonal antibody (Product # PA1-16806) followed by using DAB with hematoxylin counterstain.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ChREBP in human colon, epithelium at a 40X magnification. Samples were incubated in ChREBP polyclonal antibody (Product # PA1-16806).