PA5-30009

antibody from Invitrogen Antibodies

Targeting: ARID3A BRIGHT, DRIL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30009

Provider

Invitrogen Antibodies

Product name

DRIL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HCT116.

Concentration

0.69 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DRIL1 Polyclonal Antibody (Product # PA5-30009). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. DRIL1 Polyclonal Antibody . DRIL1 Polyclonal Antibody (Product # PA5-30009) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DRIL1 in paraformaldehyde-fixed HeLa cells using a DRIL1 polyclonal antibody (Product # PA5-30009) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DRIL1 Polyclonal Antibody detects DRIL1 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DRIL1 Polyclonal Antibody (Product # PA5-30009) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DRIL1 Polyclonal Antibody detects DRIL1 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DRIL1 Polyclonal Antibody (Product # PA5-30009) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DRIL1 Polyclonal Antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 µg of DRIL1 Polyclonal Antibody (Product # PA5-30009) 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 Polyclonal Antibody (Product # PA5-30009) diluted at 1:1,000.