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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-203-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-3 and recombinant rat IL-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo.
Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.
Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Li G, Li X, Wu H, Yang X, Zhang Y, Chen L, Wu X, Cui L, Wu L, Luo J, Liu XY
Blood cancer journal 2014 Mar 21;4:e194
Blood cancer journal 2014 Mar 21;4:e194
Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid.
Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA
Blood 2008 Nov 1;112(9):3762-71
Blood 2008 Nov 1;112(9):3762-71
Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Bartemes KR, Cooper KM, Drain KL, Kita H
The Journal of allergy and clinical immunology 2005 Oct;116(4):827-35
The Journal of allergy and clinical immunology 2005 Oct;116(4):827-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 Antibody. Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-203-NA). The ND50 is typically 0.05-0.25 µg/mL.
