Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF2524 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat IL-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-3 and less than 1% cross-reactivity with recombinant human IL-3 is observed.
- Reactivity
- Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Rat IL-3 Antibody. Recombinant Rat IL-3 (Catalog # 2524-RL) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524). The ND50 is typically 0.75-3 µg/mL.