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antibody from Invitrogen Antibodies
Targeting: IL3
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
ELISA
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- Antibody Data
- Antigen structure
- References [4]
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- Other assay [2]
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- Product number
- AHC0832 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-3 Monoclonal Antibody (653A10B1 1G8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is recommended as a capture antibody in Sandwich ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 653A10B1 1G8
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity.
Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands.
Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells.
Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system.
Seo W, Shimizu K, Kojo S, Okeke A, Kohwi-Shigematsu T, Fujii SI, Taniuchi I
Nature communications 2020 Mar 26;11(1):1562
Nature communications 2020 Mar 26;11(1):1562
Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands.
Lu Y, Xue Q, Eisele MR, Sulistijo ES, Brower K, Han L, Amir el-AD, Pe'er D, Miller-Jensen K, Fan R
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 17;112(7):E607-15
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 17;112(7):E607-15
Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells.
Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA
Immunology 2006 Jul;118(3):353-60
Immunology 2006 Jul;118(3):353-60
Molecular cloning, expression, purification, and characterization of soluble full-length, human interleukin-3 with a baculovirus-insect cell expression system.
Ding H, Griesel C, Nimtz M, Conradt HS, Weich HA, Jäger V
Protein expression and purification 2003 Sep;31(1):34-41
Protein expression and purification 2003 Sep;31(1):34-41
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Ccl5 expression from T cells is repressed by RUNX/CBFbeta complexes. a Expression profiles assessed by ELISA of CCL3, CCL4, and CCL5 and the selected cytokines IL-3, IL-4, and IFNgamma in supernatants of in vitro-stimulated CD4 + and CD8 + T cells at 5 days after stimulation. A summary of three independent measurements on three mice (with their genotypes indicated) are shown. Error bars indicate Mean +- SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student's t tests and is presented as follows: * p < 0.05, ** p < 0.01. b Flow cytometry analysis of total splenocytes and splenic TCRbeta + T cells in mice with their genotypes indicated. One representative profile of the CCL5 expression is shown in the histograms and dot plots. Numbers in the dot plots indicate the percentage of cells in each quadrant. Error bars indicate Mean +- SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student's t tests and is presented as follows: ** p < 0.01, **** p < 0.0001. Source data are provided as a Source Data file.
