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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB403-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IL-3 Antibody
- Antibody type
- Monoclonal
- Description
- IL-3
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Mouse IL-3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-3 Monoclonal Antibody (Catalog # MAB403). The ND50 is typically 0.05-0.15 µg/mL.
