Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-403-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-3 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse IL-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat IL-3 is observed and less than 1% cross-reactivity with recombinant human IL-3 is observed. Neutralizes the biological activity of recombinant mouse IL-3, but will not neutralize the biological activity of recombinant human IL-3.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01586
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Mouse IL-3 Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-3 Polyclonal Antibody (Catalog # AB-403-NA). The ND50 is typically 0.015-0.025 µg/mL.