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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF2524 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat IL-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-3 and less than 1% cross-reactivity with recombinant human IL-3 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P97688- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-3 in Rat Splenocytes. IL-3 was detected in immersion fixed rat splenocytes using Goat Anti-Rat IL-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Rat IL-3 Antibody. Recombinant Rat IL-3 (Catalog # 2524-RL) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524). The ND50 is typically 0.75-3 µg/mL.
