Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB603R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha , rhIL-1 beta , recombinant mouse (rm) IL-1 beta , rhIL-2, rhIL-3 R alpha , rhIL-3 R beta , rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF-beta 1, porcine TGF-beta 1, rhTGF-beta 1, porcine TGF-beta 2, porcine TGF-beta 1.2, rhTNF-alpha , or rhTNF-beta was observed. Does not neutralize the biological activity of rmIL-3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08700
- Isotype
- IgG
- Antibody clone number
- 4815R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tumour necrosis factor-α regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-κB and AP-1.
Kankaanranta H, Ilmarinen P, Zhang X, Adcock IM, Lahti A, Barnes PJ, Giembycz MA, Lindsay MA, Moilanen E
PloS one 2014;9(2):e90298
PloS one 2014;9(2):e90298
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 Antibody. Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Recombinant Monoclonal Antibody (Catalog # MAB603R). The ND50 is typically 0.1-0.3 µg/mL.