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LearnMA5-23808
antibody from Invitrogen Antibodies
Targeting: IL3
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Western blot
Immunocytochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- MA5-23808 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-3 Monoclonal Antibody (4806)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-3 is observed. This antibody does not neutralize the biological activity of recombinant mouse IL-3. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4806
- Vial size
- 100 μg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL-3 in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-3 monoclonal antibody (Product # MA5-23808) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-3 in TF-1 human erythroleukemic cell line. Samples were incubated in IL-3 monoclonal antibody (Product # MA5-23808). Recombinant Human IL-3 stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Monoclonal Antibody. The ND50 is typically 0.03-0.08 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-3 in TF-1 human erythroleukemic cell line. Samples were incubated in IL-3 monoclonal antibody (Product # MA5-23808). Recombinant Human IL-3 stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Monoclonal Antibody. The ND50 is typically 0.03-0.08 µg/mL.
