Explore
Validate
LearnSTJA0003214
antibody from St John's Laboratory
Targeting: IL3
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- STJA0003214 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-IL-3 antibody (STJA0003214)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-IL-3 is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Lyophilized antibody is stable at-20°C for up to 1 year from date of receipt. Upon reconstitution, store in aliquots at 2-8°C for one month or at-20°C for 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed. Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C. Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C. After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again. Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0. 2 um PVDF membrane. After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7. 5, 500 mM NaCl, 0. 1% (v/v) Tween 20, the membrane was incubated with primary antibody ( at 4°C overnight. Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies. Enhanced chemiluminescence (ECL) reagents were used to visualize
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
