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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 500606 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#500606, RRID:AB_2123712
- Product name
- PE anti-human IL-3
- Antibody type
- Monoclonal
- Antigen
- Yeast-expressed, recombinant human IL-3
- Description
- IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then stained with CD4 PE/Cy5 and BVD3-1F9 PE
- Conjugate
- Yellow dye
