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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-42743 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42743, RRID:AB_2261044
- Product name
- Rabbit Polyclonal GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GM-CSF Antibody [NBP1-42743] - Analysis of a band 14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer.
