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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Other assay [2]
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- Product number
- M500A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GM-CSF Monoclonal Antibody (3092)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M500A targets Human GM-CSF in ELISA, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 3092
- Concentration
- 1.0 mg/mL
Submitted references Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-β.
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Identification of functionally distinct domains of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies.
Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins.
Yakimchuk K, Roura-Mir C, Magalhaes KG, de Jong A, Kasmar AG, Granter SR, Budd R, Steere A, Pena-Cruz V, Kirschning C, Cheng TY, Moody DB
European journal of immunology 2011 Mar;41(3):694-705
European journal of immunology 2011 Mar;41(3):694-705
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18
Experimental lung research 2002 Jan-Feb;28(1):1-18
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18
Experimental lung research 2002 Jan-Feb;28(1):1-18
Identification of functionally distinct domains of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies.
Kanakura Y, Cannistra SA, Brown CB, Nakamura M, Seelig GF, Prosise WW, Hawkins JC, Kaushansky K, Griffin JD
Blood 1991 Mar 1;77(5):1033-43
Blood 1991 Mar 1;77(5):1033-43
Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins.
Kanakura Y, Druker B, Cannistra SA, Furukawa Y, Torimoto Y, Griffin JD
Blood 1990 Aug 15;76(4):706-15
Blood 1990 Aug 15;76(4):706-15
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of human GM-CSF was performed using a Human GM-CSF Colorimetric ELISA kit (Product # EHGMCSF) by loading 50 µL per well of a mouse anti-human GM-CSF biotinylated antibody (Product # M501B) at a concentration of 0.05 µg/mL and 50 µL of human GM-CSF Recombinant Protein (Product # SGMCSF) at 600, 240, 96, 38.4, 15.4, and 0 pg/mL (both in quadruplicate) across a 9 µg/mL mouse anti-human GM-CSF (Product # M500A) pre-coated plate. The plate was incubated for 3 hours at room temperature, washed, and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:2000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
