M500A

antibody from Invitrogen Antibodies

Targeting: CSF2 GM-CSF, GMCSF

Western blot (Recommended by provider)

ELISA (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M500A

Provider

Invitrogen Antibodies

Product name

GM-CSF Monoclonal Antibody (3092)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

M500A targets Human GM-CSF in ELISA, and WB applications and shows reactivity with Human samples. The M500A immunogen is glycosylated, CHO-derived recombinant human GM-CSF. M500A detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa. The M500A GMCSF antibody (clone 3092) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M501B (biotinylated conjugate of clone 1089). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Antibody M500A (clone 3092) and biotinylated antibody M501B (clone 1089) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3092

Vial size

500 µg

Concentration

1.0 mg/mL

Storage

-20°C

References

Submitted references

Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-β.

Yakimchuk K, Roura-Mir C, Magalhaes KG, de Jong A, Kasmar AG, Granter SR, Budd R, Steere A, Pena-Cruz V, Kirschning C, Cheng TY, Moody DB
European journal of immunology 2011 Mar;41(3):694-705

---

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18

---

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18

---

Identification of functionally distinct domains of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies.

Kanakura Y, Cannistra SA, Brown CB, Nakamura M, Seelig GF, Prosise WW, Hawkins JC, Kaushansky K, Griffin JD
Blood 1991 Mar 1;77(5):1033-43

---

Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins.

Kanakura Y, Druker B, Cannistra SA, Furukawa Y, Torimoto Y, Griffin JD
Blood 1990 Aug 15;76(4):706-15

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of human GM-CSF was performed using a Human GM-CSF Colorimetric ELISA kit (Product # EHGMCSF) by loading 50 µL per well of a mouse anti-human GM-CSF biotinylated antibody (Product # M501B) at a concentration of 0.05 µg/mL and 50 µL of human GM-CSF Recombinant Protein (Product # SGMCSF) at 600, 240, 96, 38.4, 15.4, and 0 pg/mL (both in quadruplicate) across a 9 µg/mL mouse anti-human GM-CSF (Product # M500A) pre-coated plate. The plate was incubated for 3 hours at room temperature, washed, and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:2000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.