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- References [3]
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- Product number
- M501B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GM-CSF Monoclonal Antibody (1089), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M501B targets Human GM-CSF in ELISA applications and shows reactivity with Human samples. The M501B immunogen is glycosylated, CHO-derived recombinant human GM-CSF. M501B detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa. The M501B GMCSF antibody (biotinylated conjugate of clone 1089) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M500A (clone 3092). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M501B (clone 1089) and antibody M500A (clone 3092) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 1089
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Borrelia burgdorferi infection regulates CD1 expression in human cells and tissues via IL1-β.
A NF-kappa B/Sp1 region is essential for chromatin remodeling and correct transcription of a human granulocyte-macrophage colony-stimulating factor transgene.
Identification of functionally distinct domains of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies.
Yakimchuk K, Roura-Mir C, Magalhaes KG, de Jong A, Kasmar AG, Granter SR, Budd R, Steere A, Pena-Cruz V, Kirschning C, Cheng TY, Moody DB
European journal of immunology 2011 Mar;41(3):694-705
European journal of immunology 2011 Mar;41(3):694-705
A NF-kappa B/Sp1 region is essential for chromatin remodeling and correct transcription of a human granulocyte-macrophage colony-stimulating factor transgene.
Cakouros D, Cockerill PN, Bert AG, Mital R, Roberts DC, Shannon MF
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 1;167(1):302-10
Journal of immunology (Baltimore, Md. : 1950) 2001 Jul 1;167(1):302-10
Identification of functionally distinct domains of human granulocyte-macrophage colony-stimulating factor using monoclonal antibodies.
Kanakura Y, Cannistra SA, Brown CB, Nakamura M, Seelig GF, Prosise WW, Hawkins JC, Kaushansky K, Griffin JD
Blood 1991 Mar 1;77(5):1033-43
Blood 1991 Mar 1;77(5):1033-43
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of human GM-CSF was performed using a Human GM-CSF Colorimetric ELISA kit (Product # EHGMCSF) by loading 50 µL per well of a mouse anti-human GM-CSF biotinylated antibody (Product # M501B) at a concentration of 0.05 µg/mL and 50 µL of human GM-CSF Recombinant Protein (Product # SGMCSF) at 600, 240, 96, 38.4, 15.4, and 0 pg/mL (both in quadruplicate) across a 9 µg/mL mouse anti-human GM-CSF (Product # M500A) pre-coated plate. The plate was incubated for 3 hours at room temperature, washed, and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:2000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Cleaved IL-1beta selectively induces group 1 CD1 protein expression. (A, B) Monocytes were treated with tri-acyl-CSK4 at the indicated concentrations for 24 h and the amount of GM-CSF (A) and IL-1beta (B) in the supernatant measured by capture ELISA. (C) Monocytes were treated with the recombinant mature IL-1beta at the concentrations indicated and the percentage of cells expressing CD1a, CD1b, CD1c or CD1d, assessed by FACS analysis. The data are expressed in a normalize form so that the channel with the most cells is designated 100% of maximal (MAX). (D) Monocytes were cultured for 24 h with the indicated dose of triacyl-SK 4 and ATP (1 mM), either alone or in combination. Total lysates were prepared and western blotted for IL-beta (left panel) or CD1a-positive cells were assessed by FACS (right panel). Data shown are representative of more than three experiments.
- Conjugate
- Biotin
