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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF1546 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine GM-CSF Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine GM-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human GM-CSF, recombinant mouse GM-CSF, recombinant feline GM-CSF, and recombinant canine IL-5 is observed and less than 0.7% cross-reactivity with recombinant porcine GM-CSF is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P48749.1- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- GM-CSF in Canine PBMCs. GM-CSF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with calcium ionomycin and PMA using Goat Anti-Canine GM-CSF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1546) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
