Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB518 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat GM-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects rat GM-CSF in ELISAs.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P48750
- Isotype
- IgG
- Antibody clone number
- 83329
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model.
Kellar RS, Lancaster JJ, Thai HM, Juneman E, Johnson NM, Byrne HG, Stansifer M, Arsanjani R, Baer M, Bebbington C, Flashner M, Yarranton G, Goldman S
Journal of cardiovascular pharmacology 2011 May;57(5):568-74
Journal of cardiovascular pharmacology 2011 May;57(5):568-74
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Rat GM-CSF Antibody. Recombinant Rat GM-CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM-CSF Monoclonal Antibody (Catalog # MAB518). The ND50 is typically 0.2-0.8 µg/mL.