Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB215-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GM-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human GM-CSF in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P04141
- Isotype
- IgG
- Antibody clone number
- 3209
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid.
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
GM-CSF promotes migration of human monocytes across the blood brain barrier.
BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis.
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Lehr S, Vier J, Häcker G, Kirschnek S
Microbes and infection 2018 May;20(5):284-292
Microbes and infection 2018 May;20(5):284-292
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Pęksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS
Nature communications 2018 Jul 13;9(1):2705
Nature communications 2018 Jul 13;9(1):2705
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Logue SE, McGrath EP, Cleary P, Greene S, Mnich K, Almanza A, Chevet E, Dwyer RM, Oommen A, Legembre P, Godey F, Madden EC, Leuzzi B, Obacz J, Zeng Q, Patterson JB, Jäger R, Gorman AM, Samali A
Nature communications 2018 Aug 15;9(1):3267
Nature communications 2018 Aug 15;9(1):3267
Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
Spampinato SF, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff RM
PloS one 2015;10(7):e0133392
PloS one 2015;10(7):e0133392
GM-CSF promotes migration of human monocytes across the blood brain barrier.
Vogel DY, Kooij G, Heijnen PD, Breur M, Peferoen LA, van der Valk P, de Vries HE, Amor S, Dijkstra CD
European journal of immunology 2015 Jun;45(6):1808-19
European journal of immunology 2015 Jun;45(6):1808-19
BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
Levescot A, Flamant S, Basbous S, Jacomet F, Féraud O, Anne Bourgeois E, Bonnet ML, Giraud C, Roy L, Barra A, Chomel JC, Turhan A, Guilhot F, Girard JP, Gombert JM, Herbelin A
Cancer research 2014 May 15;74(10):2669-76
Cancer research 2014 May 15;74(10):2669-76
A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis.
Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E
Cancer cell 2014 May 12;25(5):605-20
Cancer cell 2014 May 12;25(5):605-20
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Weber GF, Chousterman BG, Hilgendorf I, Robbins CS, Theurl I, Gerhardt LM, Iwamoto Y, Quach TD, Ali M, Chen JW, Rothstein TL, Nahrendorf M, Weissleder R, Swirski FK
The Journal of experimental medicine 2014 Jun 2;211(6):1243-56
The Journal of experimental medicine 2014 Jun 2;211(6):1243-56
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra CM, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Aróstegui JI, Juan M, Yagüe J, Merad M, Fagarasan S, Cerutti A
Nature immunology 2014 Apr;15(4):354-364
Nature immunology 2014 Apr;15(4):354-364
The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
The American journal of pathology 2010 Nov;177(5):2472-82
The American journal of pathology 2010 Nov;177(5):2472-82
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Hellman C, Halldén G, Hylander B, Lundahl J
Clinical and experimental immunology 2003 Jan;131(1):75-81
Clinical and experimental immunology 2003 Jan;131(1):75-81
Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
Cytokine 2003 Aug 21-Sep 7;23(4-5):126-32
Cytokine 2003 Aug 21-Sep 7;23(4-5):126-32
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
Blood 2002 Jan 1;99(1):224-31
Blood 2002 Jan 1;99(1):224-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Human GM-CSF Antibody. Recombinant Human GM-CSF (Catalog # 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND50 is typically 0.075-0.45 µg/mL.