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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF987 - Provider product page
- Provider
- R&D Systems
- Product name
- Feline GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects feline GM-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse GM-CSF, recombinant rat GM-CSF, and recombinant human GM-CSF is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-alpha plasmids preserves global CD4 T lymphocyte function after challenge with FIV.
Isolation, expression and bioactivity of feline granulocyte-macrophage colony-stimulating factor.
Maksaereekul S, Dubie RA, Shen X, Kieu H, Dean GA, Sparger EE
Vaccine 2009 Jun 8;27(28):3754-65
Vaccine 2009 Jun 8;27(28):3754-65
Isolation, expression and bioactivity of feline granulocyte-macrophage colony-stimulating factor.
Dunham SP, Bruce J
Gene 2004 May 12;332:97-106
Gene 2004 May 12;332:97-106
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Feline GM-CSF Antibody. Recombinant Feline GM-CSF (Catalog # 987-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline GM-CSF (50 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF987). The ND50 is typically 0.5-2.0 µg/mL.
