Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-415-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse GM-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human GM-CSF and recombinant rat GM-CSF is observed, and less than 3% cross-reactivity with recombinant feline GM-CSF, recombinant porcine GM-CSF, and recombinant canine GM-CSF is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14AD9
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Induction of differentiation in a B lymphoma X B lymphocyte hybrid line. II. Intraclonal heterogeneity in growth, secretion of IgM, and cytokine production in response to lipopolysaccharide.
Little J, Alling DW, Asofsky R
Journal of immunology (Baltimore, Md. : 1950) 1993 Mar 15;150(6):2129-38
Journal of immunology (Baltimore, Md. : 1950) 1993 Mar 15;150(6):2129-38
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Mouse GM-CSF Antibody. Recombinant Mouse GM-CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM-CSF Polyclonal Antibody (Catalog # AB-415-NA). The ND50 is typically 0.01-0.05 µg/mL.