Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF518 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat GM-CSF in direct ELISAs and Western blot. In direct ELISAs, approximately 100% cross-reactivity with recombinant feline GM-CSF and recombinant porcine GM-CSF is observed, approximately 30% cross-reactivity with recombinant mouse GM-CSF, recombinant canine GM-CSF and recombinant human GM-CSF is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P48750
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Rat GM-CSF Antibody. Recombinant Rat GM-CSF induces proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by rrGM-CSF is neutralized (green line) by increasing concentrations of Goat Anti-Rat GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF518). The ND50 is typically 0.1-0.6 ug/mL.