Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF711 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects porcine GM-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat GM-CSF is observed, 15% cross-reactivity with recombinant human GM-CSF is observed, and 5% cross-reactivity with recombinant mouse GM-CSF is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q29118
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Porcine GM-CSF Antibody. Recombinant Porcine GM-CSF (Catalog # 711-PG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM-CSF (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine GM-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF711). The ND50 is typically 0.2-1.0 µg/mL.