ABIN2508224

antibody from antibodies-online

Targeting: CSF2 GM-CSF, GMCSF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2508224

Provider

antibodies-online

Product name

anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibody (Biotin)

Antibody type

Polyclonal

Antigen

Other

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

50 μg

Storage

-20°C

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.