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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB16920 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16920, RRID:AB_10678329
- Product name
- CSF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CSF2.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.
Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.
Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.
Kaushansky K, O'Hara PJ, Berkner K, Segal GM, Hagen FS, Adamson JW
Proceedings of the National Academy of Sciences of the United States of America 1986 May;83(10):3101-5
Proceedings of the National Academy of Sciences of the United States of America 1986 May;83(10):3101-5
Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor.
Cantrell MA, Anderson D, Cerretti DP, Price V, McKereghan K, Tushinski RJ, Mochizuki DY, Larsen A, Grabstein K, Gillis S
Proceedings of the National Academy of Sciences of the United States of America 1985 Sep;82(18):6250-4
Proceedings of the National Academy of Sciences of the United States of America 1985 Sep;82(18):6250-4
Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.
Lee F, Yokota T, Otsuka T, Gemmell L, Larson N, Luh J, Arai K, Rennick D
Proceedings of the National Academy of Sciences of the United States of America 1985 Jul;82(13):4360-4
Proceedings of the National Academy of Sciences of the United States of America 1985 Jul;82(13):4360-4
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Csf2 polyclonal antibody (Cat # PAB16920) shows detection of a band ~15 KDa in size corresponding to recombinant human Csf2. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
